Title of article :
Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer
Author/Authors :
Arefi ، Mohammad Department of Medical Biotechnology - School of Advanced Technologies in Medicine - Golestan University of Medical Sciences , Abdollahi ، Abbas Department of General Surgery - School of Medicine, Surgical Oncology Research Center, Ghaem Hospital - Mashhad University of Medical Sciences , Khosravi ، Ayyoob Department of Molecular Medicine - School of Advanced Technologies in Medicine - Golestan University of Medical Sciences , Moradi ، Abdolavahab Department of Microbiology - School of Medicine, Research Center of Gastroenterology and Hepatology - Golestan University of Medical Sciences , Aghaee-Bakhtiari ، Hamid Department of Medical Biotechnology - School of Medicine - Mashhad University of Medical Sciences , Javid ، Najmeh Department of Microbiology - School of Medicine, Research Center of Gastroenterology and Hepatology - Golestan University of Medical Sciences , Evazalipour ، Mehdi Department of Pharmaceutical Biotechnology - School of Pharmacy - Guilan University of Medical Sciences , Kianmehr ، Anvarsadat Department of Medical Biotechnology - Medical Cellular and Molecular Research Center, School of Advanced Technologies in Medicine - Golestan University of Medical Sciences
Abstract :
Background and objectives: Colorectal cancer (CRC) is one of the leading causes of cancerrelated mortality in the world. MicroRNAs (miRNAs) have potential as diagnostic biomarkers for various diseases including cancer. This study was undertaken to investigate expression of miR21 before and after surgery in patients with hereditary CRC. Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR21 in serum of subjects was evaluated using realtime PCR, along with two reference genes, let7d and let7g. The realtime expression results and Ct values were collected and analyzed based on the 2∆∆ct method. Results: In spite of tumor removal, serum miR21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0.022). Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.
Keywords :
Colorectal cancer , Gene Expression , Real , Time Polymerase Chain Reaction
Journal title :
Medical Laboratory Journal
Journal title :
Medical Laboratory Journal
