Author/Authors :
Rohani ، Razieh Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences and Health Services (SBMU) , Aliaghaei ، Abbas Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences and Health Services (SBMU) , Abdollahifar ، Mohammad-Amin Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences and Health Services (SBMU) , Sadeghi ، Yousef Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences and Health Services (SBMU) , Zare ، Leila Department of Physiology - Faculty of Medical Sciences - Tarbiat Modares University , Dehghan ، Samaneh Department of Physiology - Faculty of Medical Sciences - Tarbiat Modares University , Heidari ، Mohammad Hassan Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences and Health Services (SBMU)
Abstract :
Objective Epilepsy is accompanied by inflammation, and the antiinflammatory agents may have antiseizure effects. In this investigation, the effect of deep brain stimulation, as a potential therapeutic approach in epileptic patients, was investigated on seizureinduced inflammatory factors. Materials and Methods In the present experimental study, rats were kindled by chronic administration of pentylenetetrazol (PTZ; 34 mg/Kg). The animals were divided into intact, sham, lowfrequency deep brain stimulation (LFS), kindled, and kindled +LFS groups. In kindled+LFS and LFS groups, animals received four trains of intrahippocampal lowfrequency deep brain stimulation (LFS) at 20 minutes, 6, 24, and 30 hours after the last PTZ injection. Each train of LFS contained 200 pulses at 1 Hz, 200 µA, and 0.1 ms pulse width. One week after the last PTZ injection, the Ymaze test was run, and then the rats’ brains were removed, and hippocampal samples were extracted for molecular assessments. The gene expression of two proinflammatory factors [interleukin6 (IL6) and tumor necrosis factoralpha (TNFα)], and glial fibrillary acidic protein (GFAP) immunoreactivity (as a biological marker of astrocytes reactivation) were evaluated. Results Obtained results showed a significant increase in the expression of of interleukin6 (IL6), tumor necrosis factor (TNF)α, and GFAP at oneweek post kindling seizures. The application of LFS had a longlasting effect and restored all of the measured changes toward normal values. These effects were gone along with the LFS improving the effect on working memory in kindled animals. ConclusionThe antiinflammatory action of LFS may have a role in its longlasting improving effects on seizureinduced cognitive disorders.
Keywords :
Deep Brain Stimulation , Epilepsy , GFAP , Interleukin6 , TNFα Cell Journal(Yakhteh) , Vol 23 , No 1 , 2021 , Pages: 8592 ,
