Optimization of biosynthesized Zn nanoparticles by poisonous Taxus baccata leaves extract and evaluation of their effect on the bacterias and MCF-7 cancer cells

Recently, phyto nanotechnology has proposed new methods for the biosynthesis of nanoparticles and is also an ecofriendly, stable, rapid, simple, and costeffective method. The present study reported the green synthesis of Zn nanoparticles (Zn NPs) by Taxus baccata extract, and the performance of them was tested against three types of bacteria and a type of cancer cells. These experiments are designed in a fully compatible environment. In order to evaluate effective parameters, Ultravioletvisible (UV–vis) spectroscopy was used. In addition we had a comparative study of the performance of synthesized nanoparticles on bacteria, and a comparative study on the pure extract of this plant as an anticancer agent along with biosynthesized Zn nanoparticles. The SEM and FESEM results revealed hexagonal structure with the particle size of 20 nm. TEM analysis of the synthesized nanoparticles showed hexagonal particles with an average size of 2025 nm. FTIR analysis confirmed the reduction of functional groups, Alkaloids, corresponding of synthsis Zn nanoparticles from their salt solution. The antibacterial activity of Taxus baccata extract, Zn nanoparticles, and zinc nitrate solution was tested against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Anticancer activity of Taxus baccata extract, zinc nitrate solution and synthesized Zn nanoparticles on cancer cells was assessed by Breast cancer cell line MCF7. The results showed that the synthesized Zn nanoparticles using plant leaf extract had strong anticancer activity on MCF7 cells. On the other hand, the synthesized metal nanoparticles had no effect on the three types of bacteria.