Title of article :
Electrophoretic removal of sodium dodecyl sulphate from peptide solution through a hydrogel-micropipette system
Author/Authors :
Soltantabar Shahabedini ، Atieh Department of R D - Research and Development Center of Iranian Tissue Product , Alinezhad Chamazketi ، Mohammad Department of Biotechnology - Malek Ashtar University of Technology (MUT) , Yaghoubi Nezhad ، Amir Department of Cellular and Molecular Biology - Faculty of Sciences - Higher Education Institute of Rab-Rashid , Mostafavi ، Mojtaba Department of Chemometrics - HiTech Institute of Theoretical and Computational Chemistry
Abstract :
SDS removal is an important process in proteomics after protein digestion. The research study evaluated an offline SDS removal, as well as, the capability of the offline electrophoretic system for SDS removal. TyrTyrTyr, GluValPhe, and Bradykinin were three peptides to be tested at the presence of the SDS. The electrophoretic system included a 20 µL glass micropipette containing sample which was immersed into acetonitrile at one end and hydrogel at another end. The SDS removal was driven by applying voltage to the system and after removal, the sample inside micropipette can be collected for liquid chromatography, capillary electrophoresis, and mass spectrometry.
Keywords :
Electrophoretic sample preparation , proteomics , offline sample preparation , sodium dodecyl sulphate
Journal title :
Eurasian Chemical Communications
Journal title :
Eurasian Chemical Communications
