Quality assessment and evaluation of Oroxylum indicum through HPLC fingerprint and QAMS for important flavonoid components

Oroxylum indicum is an important Ayurvedic medicinal plant used in medicinal formulations to treat many diseases. In this research, an effective, sensitive, reliable, cost effective and comprehensive assessment of quality of Oroxylum indicum has been developed based on high performance liquid chromatography (HPLC) fingerprint analysis combined with the quantitative analysis of multicomponents by a single marker (QAMS) method. The contents of four components i.e., Scutellarin, Hispidulin, Baicalein and BiochaninaA, has been determined, simultaneously, and Baicalein is used as internal reference standard. It was established that there is no major difference between the QAMS method and the traditional external standard method (ESM) (RSD 2.00%). This signifies that QAMS is a consistent and expedient method for the content determination of multiple components, particularly when there is nonavailability of multiple reference standards. This method was also validated in terms of linearity, precision, stability, recovery and reproducibility. Hence it can be effectively applied for quality assessment of Oroxylum indicum in various Ayurvedic formulations.