Author/Authors :
Yazdani, R. isfahan university of medical sciences - School of Medicine - Dept of Microbiology, اصفهان, ايران , Oshaghi, M. Laboratory Sciences Research and Educational Center - Microbiology unit, ايران , Havayi, A. Laboratory Sciences Research and Educational Center - Microbiology unit, ايران , Pishva, E. Laboratory Sciences Research and Educational Center - Microbiology unit, ايران , Salehi, R. isfahan university of medical sciences - School of Medicine - Dept of Genetics, اصفهان, ايران , Sadeghizadeh, M. tarbiat modares university - Dept of Genetics, تهران, ايران , Foroohesh, H. iran university of medical sciences - School of Medicine - Dept of Microbiology, تهران, ايران
Abstract :
Wound infections are a common cause of staphylococcal infections. An ability of Saureus is to adhere and form biofilm on host surfac es . Bio film is an exopolysaccharide, a slime matrix around mult iple layers of cells and is mediated by expression of the ieaADBC operon. The present study evaluated the biofilm forming capac ity and the presence of icaAD gene among Siaureus isol ated from wound infections. Slime production assay was performed by cultiva tion on Congo Red Agar plate . In addition, Quantitative biofilm formation determined by microtiter plate assay PCR method used for detection of icaAD gene. Fifty strains were identified, 54% of the isolates produced black colonies on CRA plate , 52% were positive biofilm forming, and all strains carried the icaAD gene. Reg arding the ability of Siaureus to form biofilms helps the bacterium to survive hostile environments within the host, suggests that biofilm production is a risk factor for infection. It is important in rapid diagno sis and treatment biofilm forming strains, because biofilm form ation may lead to increased antimicrobial resistance and create a significant impediment to wound healin g.
