The Effect of CaNa2-EDTA on Metabolism of Zinc and Carbohydrate as well as Some Biochemical Factors in Experimental Diabetes

Background: To investi gate the effect of CaNa2-EDTA and experimental diab etes (lODM) on zinc and carbohydrate metabolism and the activities of GOT, GPT and alkaline phosphatase. Methods: Forty male norm al albino (Wist ar) rats of 8 weeks of age were fed with a basal diet. Twenty rats were then intraperitoneally injected with alloxan to induce diabetes. Then after one week ten rats from each group (n= 20) were admini strated intraperitoneally with CaNa 2-EDTA for further three weeks. Body weight gain and food intake were recorded regularly. On day 2 1 animals were killed and blood gluco se, serum and tissues zinc concentrations, liver glycogen contents, serum transaminase enzymes (GOT, GPT) , and serum alkaline phosphatase activities were determined. Results: The administration of CaNa2-EDTA signifi cantly altered body weight gain, food intak e and serum zinc concentration of either diabetic or non-diabet ic animals. Both diabetic and non-diabetic rats given CaNa2-EDTA had higher blood glucose than their controls. Liver glycogen was also found to be higher in CaNa2-EDTA non-diabetic rats than their controls. CaNa2-EDTA also led to increa sing of GOT and GPT, and decreasing serum alkaline phosphatase. Conclusion: To conclude, the present study demonstrates that CaNa2-EDTA had an effect on the previous parameters. Therefore it was appeared that CaNa2-EDTA resulted in the development of severe diabetes.