Author/Authors :
Rahimi, H. R. tehran university of medical sciences tums - School of Public Health and Institute of Public Health Research, - Dept of Medical Parasitology Mycology, تهران, ايران , Kia, E. B. tehran university of medical sciences tums - School of Public Health and Institute of Public Health Research, - Dept of Medical Parasitology Mycology, تهران, ايران , Mirhendi, S. H. tehran university of medical sciences tums - School of Public Health and Institute of Public Health Research, - Dept of Medical Parasitology Mycology, تهران, ايران , TLEBI, a. isfahan university of medical sciences, اصفهان, ايران , Fasihi Harandi, M. kerman university of medical sciences - Dept ofMedical Parasitology Mycology, كرمان, ايران , Jalali-zand, N. tehran university of medical sciences tums - School of Public Health and Institute of Public Health Research, - Dept of Medical Parasitology Mycology, تهران, ايران , Rokni, M. B. tehran university of medical sciences tums - School of Public Health and Institute of Public Health Research, - Dept of Medical Parasitology Mycology, تهران, ايران
Abstract :
Background: Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. Using DNA based methods for strain /genotype characterizations of E. granu/osus have some difficulties, especially access to an efficient and pure concentration of DNA and proper primers. Methods:Using grinder method , a pure and high concentration DNA was extracted from 10 human hydatid cysts collected from Isfahan (central Iran) hospitals, and processed for peR reaction. Results: Using DNASIS, the primers were designed in internal transcribed spacer 1 (ITS I) region, following analysis of 30 E. granu/osus nucleotide sequences, extracted from gene bank. Conclusion: Thi s new and specific E. granulosus primer which amplified DNA thoroughly can be applied for molecular studies on echinococcosis.
Keywords :
Echinococcus granu!osus , peR , Prim er design , DNA , ITS1 , DNASIS
