Title of article :
Using Comet Assay for Genotoxic Evaluation of Zataria multiflora Boiss., An Iranian Endemic Plant
Author/Authors :
Ayubi ، Z. Department of Medical Parasitology and Mycology - Afzalipoor Medical School - Kerman Medical University , Jafari ، A.A. Department of Medical Parasitology and Mycology - Medical School - Shahid Sadougi University of Medical Sciences , Ayatollahi-Mousavi ، S.A. Department of Medical Parasitology and Mycology - Afzalipoor Medical School - Kerman Medical University , Jafari ، H. Department of Food and Drug Control - School of Pharmacy - Tehran University of Medical Sciences , Panddeh ، M. Department of Biochemistry - School of Medicine - Shahid Sadoughi University of Medical Sciences
Abstract :
Background: Zataria multiflora Boiss. (Avishane Shirazi), as an Iranian endemic plant, belongs to the Lamiaceae family and may be used as a food preservative. This study aimed to detect potential genotoxic effects of Z. multiflora extract. Methods: Hydroalcoholic extract of Z. multiflora was prepared. Human B lymphocytes were treated with 1% extract within 3 and 24 h. Sterile Phosphate Buffered Saline (PBS) and cisplatin were used as negative and positive controls, respectively. DNA damage profiles were examined using comet assay (Single Cell Gel Electrophoresis). Data were statistically analyzed by SPSS software v. 21. Results: No statistically significant (p=0.071) DNA damage was observed in B lymphocytes treated with either Z. multiflora extract or PBS after 3 and 24 h. However, there was a statistically significant difference (p=0.0001) between DNA damage in B lymphocytes that treated with cisplatin and Z. multiflora after 3 and 24 h. Conclusion: The comet assay used in the current study showed that Z. multiflora had no genotoxic effect. DOI: 10.18502/jfqhc.8.1.5463
Keywords :
Thymus Plant , Comet Assay , Mutagenicity Tests , DNA Damage , Food Safety
Journal title :
Journal of Food Quality and Hazards Control
Journal title :
Journal of Food Quality and Hazards Control
