Author/Authors :
Pawade, Mrunalini M. Department of Microbiology - KNP, College of Veterinary Science, Shirwal , Bhosale, Ashok V. Department of Microbiology - College of Veterinary Science, Udgir, India , Mhase, Prashant P. Department of Microbiology - KNP, College of Veterinary Science, Shirwal , Kulkarni, Mahesh B Department of Microbiology - College of Veterinary Science, Udgir, India , Budhe, Shridhar D Department of Microbiology - College of Veterinary Science, Udgir, India
Abstract :
The presence of Salmonella spp in collected faecal samples was assessed by performing the pre-enrichment and enrichment culture, followed by PCR assay. The primer was selected from the invA gene, specific for the detection of Salmonella spp. It was observed that 28% (10/35) of Salmonella were isolated by the conventional method. All Salmonella isolates thus obtained were subjected to PCR for the invA gene and all were found positive. In order to provide a more accurate profile of the prevalence of Salmonella spp in faecal samples, it was pertinent to use invA gene specific PCR method that could be considered as rapid technique for identification of Salmonella spp.
Keywords :
Salmonella , InvA gene , Polymerase chain reaction (PCR) , Faecal and Simmon citrate agar
