Effects of different concentrations of DMSO and glycerol on cryopreservation of Trichomonas gallinae

Conventional methods for the propagation and preservation of parasites in vivo or invitro have limitations, which include the need for labor, the initial isolation and loss of strains,bacterial and fungal contamination, and changes in the original biological and metaboliccharacteristics of the organism. All of these disadvantages are reduced considerably bycryopreservation. In this study, we examined the effects of different concentrations of dimethylsulfoxide (DMSO) and glycerol on the survival rate of . Both of the twocryoprotectants examined at different concentrations showed a protective effect of .WhenDMSOwas used as the cryoprotectant, a maximum survival rate (70%) was achieved at aconcentration of 10% over a short-term duration of freezing (24 h). Glycerol at a concentrationof 10% had the highest effect on cryoprotectivity after 70 days, while DMSO 20% showed thelowest cryopreservation for In conclusion, DMSO and glycerol are appropriateprotective materials for the cryopreservation of . The solutions of DMSO 10% andglycerol 10% could be the best choice of cryoprotectant for short-term (24 hours) and long-term(70 days) protection, respectively.