Compensatory ovarian changes, mast cell distributionand luminal structure changes followingunilateral ovariectomy in rats

To follow-up the present study, 40 mature female rats were used. The animals were divided into test,control and sham groups. The rats in the test group were laparotomized and unilateral ovariectomy was done.On the 1st and 2nd met-oestrous after operation, the tissue samples were dissected out and processed forhistological study. The intact ovary in the test group underwent a significant (P 0.05) hypertrophy andcompensatory changes including double angiogenesis, high cytoplasmic vacuolation in granulosa cell typesof corpus luteum, compensatory follicular development, decreasing of follicular atresia, approximatelydouble ovulation rate and increase in the width of cortex and medulla. The uterine horn on the intact side wasapproximately similar to the control and sham, while morphometric analyses showed that the horn on theovariectomized side had significant decrease in the thickness of different layers. Scattering of endometrial glands on the uterine horn of the intact side was normal, showing a dense basophilic appearance inhaematoxyline and eosin staining sections. The horn on the ovariectomized side showed low gland scattering.Histomorphometric analyses showed no significant differences between the vaginas in the three differentgroups. Distribution of mast cells (MCs) as essential cells participating in angiogenesis was investigated. Inthe test group, mast cells were considerably increased in number around the blood vessels in the medulla ofthe intact ovary and intact uterine horn. Mast cells were very low in number on the horn on theovariectomized side.