Title of article
Identification of excretory-secretory products from larval stages of Ostertagia circumcincta cultured in vitro by SDS-PAGE and immunoblotting
Author/Authors
Alborzi, A.R shahid chamran university of ahvaz - School of Veterinary Medicine - Department of Pathobiology, اهواز, ايران , Seyfiabad Shapouri, M.R shahid chamran university of ahvaz - School of Veterinary Medicine - Department of Pathobiology, اهواز, ايران , Hoghooghi Rad, N islamic azad university - School of Veterinary Medicine Specialized Sciences - Department of Parasitology, ايران
From page
13
To page
16
Abstract
Exsheathing fluid (EF) and excretory-secretory products (ES) of infective third-stage cultured larvae of Ostertagia circumcincta were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Five and seven predominant proteins were found in the EF and ES products, respectively. Immunoblotting by sheep pre-infection serum did not react with any of the EF and ES proteins, but the post-infection serum recognized four proteins of 44.5, 41.5, 38 and 24 kDa of the ES products. None of the EF products was recognized by the post-infection serum. Protectivity of the four proteins remains to be determined.
Keywords
Ostertagia circumcincta , ES products , Sheep , SDS , PAGE , Immunoblotting
Journal title
Iranian Journal of Veterinary Research (IJVR)
Journal title
Iranian Journal of Veterinary Research (IJVR)
Record number
2582365
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