Author/Authors :
Waheed, Abdul Hazara University - Genetics Department, Pakistan , Ahmad, Habib Hazara University - Department of Genetics, Pakistan , Abbasi, Fida M. Hazara University - Genetics Department, Pakistan , Hamid, F.S. National Tea Research Institute Shinkiari, Pakistan , Shah, A.H. Hazara University - Genetics Department, Pakistan , Safi, Fayaz Ahmad Hazara University (KPK) - Genetics Department, Pakistan , Ali, Hamid Hazara University - Department of Genetics, Pakistan
Abstract :
Study was conducted at Genetics department Hazara University, Pakistan for the determination of pollen sterility and fertility. Bas-385, Oryza rufipogon, Bas-385 X Oryza rufipogon were used for experiment. Pollen sterility was determined from 1000 pollen grains from parents and F1 hybrids. The spikelet was collected near anthesis in 70 % ethanol. The pollen grains were stained in 1 % I2 KI solution. Darkly stained and round pollen grains were counted as fertile while lightly stained and shrivelled were counted as sterile. Out of over 1000 pollen grains, 851 were darkly stained and were counted as fertile, whereas 149 remain unstained and were considered as sterile. Pollen sterility of Oryza rufipogon was thus 4.6 %. The average spikelet sterility of Oryza rufipogon was 44.3 %, indicating that this specie was partly sterile although it showed high pollen fertility
