Rapid in vitro multiplication of Cistus ladanifer L. var. maculates Dun.

This work aimed at establishing the protocol for plant regeneration of Cistus ladaniferus L.var. maculatus Dun. via in vitro micro propagation. The nodal explants used came either from cuttings already cultured in media from seed germination or from mature trees. The latter were pre-treated with an antioxidant solution (ascorbic acid 100 mg/L and citric acid 150 mg/L) before sterilization. Explants were cultured on Murashige and Skoog medium (MS) supplemented with 30 g/L sucrose, 8 g/L agar and different concentrations of cytokines (6-Benzylaminopurine (BAP) or(6-(3-methyl-2- butenylamino) purine (2iP)) or of auxins (NAA or IBA) or of their combination. MS medium supplemented with 0,2 mg/L BAP gave the best caulogenic response and the best proliferation of shoots with an average of 1,46 shoots per explants developed on 62,5 % of the cultures after 4 weeks. In vitro regeneration shoots developed roots directly from the basal cut ends of shoots without an intervening callus phase, MS medium supplemented with 0.1 mg/L indol butyrique acid (IBA) after 30 days of incubation, 50 % shoots developed roots.In the stage of acclimatization, 70 % of the vitro-plants were able to survive in the mixture of garden soil and vermiculite (2:1).In the present study, we established an effective and reproducible protocol for the multiplication of C. ladanifer. This study is a basis for the valuing of this medicinal plant.