Author/Authors :
el hamdani, n. university chouaïb 1 doukkali - faculty of sciences - laboratory of bioorganic chemistry, department of chemistry, El Jadida, Morocco , filali-ansari, n. faculty of sciences - department of biology, laboratory of biochemistry, nutrition and valorization of natural resources, El Jadida, Morocco , zefzoufi, m. university chouaïb 1 doukkali - faculty of sciences - laboratory of bioorganic chemistry, department of chemistry, El Jadida, Morocco , derhali, s. university chouaïb 1 doukkali - faculty of sciences - department of bioorganic chemistry, department of chemistry, El Jadida, Morocco , el abbouyi, a. faculty of sciences - laboratory of biochemistry, nutrition and valorization of natural resources, department of biology, El Jadida, Morocco , mouzdahir, a. university chouaïb 1 doukkali - faculty of sciences - laboratory of bioorganic chemistry, department of chemistry, El Jadida, Morocco , sraidi, k. university chouaïb 1 doukkali - faculty of sciences - laboratory of bioorganic chemistry, department of chemistry, El Jadida, Morocco , harjane, t. university chouaïb 1 doukkali - faculty of sciences - laboratory of bioorganic chemistry, department of chemistry, El Jadida, Morocco , fdil, r. university chouaïb 1 doukkali - faculty of sciences - laboratory of bioorganic chemistry, department of chemistry, El Jadida, Morocco , el khyari, s. faculty of sciences - laboratory of biochemistry, nutrition and valorization of natural resources, department of biology, El Jadida, Morocco
Abstract :
The present study was designed to evaluate the phytochemical profile and potential antibacterial activity of R. monosperma aerial parts extracts. The aerial parts extracts showed the presence of alkaloids, terpenoids, tannins, saponins, flavonoids and coumarines. In addition, total phenols, flavonoids as well as tannins contents were determined in four organic extracts from stems, flowers and seeds of R. monosperma and used to assess their contribution to a potential antibacterial effect. Stems and seeds methanol extracts exhibited high total phenols content (495.75 mg GAE/g and 195.69 mg GAE/g) respectively. In contrast, the methanol extract of the flowers showed lower total phenolic content (146.77 mg GAE/g) but higher tannins content (381.92 mg CAE/g). When compared to total phenols and tannins contents, the aerials parts of R. monosperma extracts showed low contents of total flavonoids (33.37 QE/g to 65.88 QE/g). The in vitro antibacterial activity of R. monosperma aerials parts extracts at a concentration of 500 mg/ml was investigated against various strains of bacteria by disc diffusion method. The ethyl acetate extracts of the stems and the flowers demonstrated a very significant activity (Ø 14mm) on Salmonella sp meanwhile, hexane and dichloromethane extracts from seeds exhibited very significant antibacterial effect against Bacillus sp and Escherichia coli.
