Effect of Chlorogenic and Caffeic Acids on Activities and Isoenzymes of G6PDH and 6PGDH of Artemisia Herba Alba Seeds Germinated for One and Three Days in Light and Dark

G6PDH and 6PGDH activities of Artemisia herba alba seeds during early stages of seed germination under conditions of light and dark were measured, in the presence of Chlorogenic or Caffeic acids; G6PDH (glucose 6 phosphate dehydrogenase) and 6PGDH (6 phospho gluconate dehydrogenase) activities were inactivated in the presence of both phenolic acids and were stimulated upon germination in the dark (Table 1 and Table 2). This novel approach provides evidence for the variable needs of pentose phosphate pathway activity, depending on concentrations of phenolic acids present and /or light conditions; moreover, a clear response of such germination condition was earlier expression of a second isoenzyme for G6PDH in the presence of 0.4 mM Chlorogenic or Caffeic acids with apparently variable inhibitory pattern.