Effects of in- vivo Application of Cold Atmospheric Plasma on the Leukocyte of Balb/c Mice

The current study included the direct effect of CAP on a number of leukocytes in the laboratory mouse by exposing the skin to CAP using the floating electrode dielectric barrier discharge (FE-DBD).The study was conducted on mice which were (2-3) months of age with the average weight of 38g. 250 mice were divided into two groups. Group I is exposed to a single dose of CAP and group II is exposed to multidose of CAP. Every group was divided depending on the exposure time and time after exposure into 5 subgroups. Exposure process has by using Floating Electrode -Dielectric Barrier Discharge for (15,30,60,120) sec and a sub-group is left as a control group. Blood samples are taken after (1, 2, 3, 7, and 14) days from exposure. Leukocytes were measured by using count blood cells device. The results revealed that CAP causes a significant decrease in number of leuckocyte after1day from exposure, then recovery was proportional with a dose of CAP and with sampling time as well. This may indicate stimulation of bonemarrow to produce more leuckocyte and eventually indicates that CAP effect was transported far from the site of exposure.