Molecular-Genetic Analysis of Cows Genetic Structure and Determination of Genealogical Relatedness Level of Bulls of Modern Dairy Breeds

As a result of molecular-genetic analysis of two cow populations – Ukrainian Brown Dairy breed (UBD) and Sumy interbreed type of Ukrainian Black-and-White breed (SITUBWD) in the technique of ISSR analysis with the use of the fragment of microsatellite loci with trinucleotide core motif (AGC)6 C (S1) as an amplification primer, there were identified 23 DNA-patterns. It was determined that genetic locus with the identified size of 1350 bp was monomorphic in animals of SITUBWD occurring in all study samples, however, in UBD breed cows the amplicons of this size were not detected at all (p<0.001). The population-genetic analysis of the two studied animal groups (taking into account maternal origin) by molecular-genetic testing in the ISSR-PCR technique revealed essential statistically significant differences. The total average number of PCR products obtained from the cows of the newly developed Sumy interbreed type of Ukrainian Black-and-White breed was 15,050 being 12,450 in Ukrainian Brown Dairy breed (p <0,001). A rather high level of genetic similarity between the animals of Ukrainian Brown Dairy and Sumy interbreed type of Ukrainian Black-and-White Dairy (0,768) is quite possibly connected with the influence of the unique aboriginal Lebedynsky breed on which maternal base they were developed.