Author/Authors :
Shehadeh, Mayadah B. University of Jordan - Faculty of Pharmacy - Department of Pharmaceutical Sciences, Jordan , Sosa, Silvio University of Trieste - Department of Materials and Natural Resources, Italy , Suaifan, Ghadeer A.R.Y. University of Jordan - Faculty of Pharmacy - Department of Pharmaceutical Sciences, Jordan , Darwish, Rula M. University of Jordan - Faculty of Pharmacy - Department of Pharmaceutics and Pharmaceutical Technology, Jordan , Giangaspero, Anna University of Salerno - Department of Pharmaceutical Sciences, Italy , Vassallo, Antonio University of Salerno - Department of Pharmaceutical Sciences, Italy , Lepore, Laura University of Salerno - Department of Pharmaceutical Sciences, Italy , Oran, Sawsan A. University of Jordan - Faculty of Science - Department of Biological Sciences, Jordan , Hammad, Hana University of Jordan - Faculty of Science - Department of Biological Sciences, Jordan , Tubaro, Aurelia University of Trieste - Department of Materials and Natural Resources, Italy , Tommasi, Nunziatina De University of Salerno - Department of Pharmaceutical Sciences, Italy , Della Loggia, Roberto University of Trieste - Department of Materials and Natural Resources, Italy
Abstract :
Inflammation is a host defense mechanism to get rid of injurious stimuli and to induce tissue healing process. In Jordan, Salvia species are traditionally used to treat inflammation and other ailments. The aim of this study was to evaluate the potential of six Salvia species grown in Jordan to inhibit cutaneous inflammation. Topical antiinflammatory activities of hexane (Hex), ethyl acetate (EtOAc) and methanol (MeOH) extracts from Salvia species aerial parts (S. ceratophylla, S. dominica, S. multicaulis, S. palaestina, S. spinosa and S. syriaca) were evaluated for the inhibition of croton oil-induced mouse ear oedema. Almost all extracts reduced oedema at the tested dose (300 μg/cm^2). Hex and EtOAc extracts exhibited the best anti-inflammatory effect in a dosedependent pattern. Dose inducing 50% oedema inhibition (ID50) in vivo was found to be in the range of 87 - 300 μg/cm^2 and 47-146 μg/cm^2 for Hex and EtOAc extracts, respectively. In comparison with indomethacin (ID50 96 μg/cm^2), S. palaestina and S. multicaulis EtOAc extracts were two folds more potent (ID50 47 and 50 μg/cm^2, respectively).Whereas, the ID50 of S. syriaca Hex extract (87μg/cm^2) was comparable to that of indomethacin. In conclusion, the results illustrated that S. multicaulis, S. palaestina and S. syriaca can be regarded as promising natural sources of anti-inflammatory drugs
Keywords :
Croton oil , Lamiaceae , Mouse , ear erythema test , Salvia , Topical anti , inflammatory activity
