Author/Authors :
Yien Lee, Chee Department of Veterinary Clinical Studies - Faculty of Veterinary Medicine - University Putra Malaysia, UPM Serdang, Selangor, Malaysia , Yee Tan, Chew Department of Veterinary Clinical Studies - Faculty of Veterinary Medicine - University Putra Malaysia, UPM Serdang, Selangor, Malaysia , Jing Tan, Yi Department of Veterinary Clinical Studies - Faculty of Veterinary Medicine - University Putra Malaysia, UPM Serdang, Selangor, Malaysia , Wai Nung Wong, Jeffrey NHK Bioscience Solutions Sdn Bhd, Kuala Lumpur, Malaysia , Mohan Jacob, Daniel Department of Veterinary Clinical Studies - Faculty of Veterinary Medicine - University Putra Malaysia, UPM Serdang, Selangor, Malaysia , Xin Lim, Jia NHK Bioscience Solutions Sdn Bhd, Kuala Lumpur, Malaysia , Ananthan, Gunaselvi NHK Bioscience Solutions Sdn Bhd, Kuala Lumpur, Malaysia , Suri Arshad, Siti Department of Veterinary Pathology and Microbiology - Faculty of Veterinary Medicine - University Putra Malaysia, UPM Serdang, Selangor, Malaysia , Thevi Selvarajah, Gayathri Department of Veterinary Clinical Studies - Faculty of Veterinary Medicine - University Putra Malaysia, UPM Serdang, Selangor, Malaysia , Toung Ooi, Peck Department of Veterinary Clinical Studies - Faculty of Veterinary Medicine - University Putra Malaysia, UPM Serdang, Selangor, Malaysia
Abstract :
This paper reports the first nearly complete genome sequence of a Malaysian Porcine bocavirus (PBoV)
detected in Malaysia from a porcine organ sample. In Malaysia, both Human bocavirus (HBoV) and PBoV have been
reported, at prevalence of 5.6% and 90.9% respectively. Most recently, in 2018, PBoV emerged as a zoonosis when a
human case of PBoV-related respiratory infection is reported in a child with history of contact with porcine secretion.
With this evidence of human infection with PBoV, considering close contact between humans and pigs in the swine
industry, further study of PBoV genetic characterization and epidemiology is warranted. This study attempts to obtain
the complete genome sequence of a Malaysian PBoV isolate detected in a pig farm in the state of Selangor. A nearly
complete sequence was obtained through primer walking and touchdown PCR method. Based on phylogenetic analysis for the nearly complete genome, the Malaysian PBoV strain is genetically characterized as PBoV G3B, sharing a
close relationship with a reference PBoV strain from U.S.A. with 98% homology. This finding may facilitate further
epidemiological studies and diagnostic development of PBoV in Malaysia.
