Effect of the Energy Drink Bison on Mitosis And Nucleic Acids Content In Vicia faba

The present investigation had been conducted to study the mutagenic effects of four concentrations of energy drink Bison (50, 80, 90, and 100%) with three exposure times (6, 12, and 24 hours) on root meristems of Vicia faba plant. Mitotic index was reduced in all treatments except for two treatments: 90% Bison with two exposure times 6 and 12 h. On the other hand, total abnormalities were highly increased in all treatments. The most common abnormalities were: Stickiness, disturbed, C-metaphase, star shape and lagging chromosomes. In addition, bridges, multipolar, breaks, (micro-multi) nuclii and dinucleated occurred with very low frequencies in some treatments. These results-indicated that Bison had Turbagenic effects. Unbalanced mitotic stages percentages occurred as a result of all treatments compaired with control. Different Bison treatments caused altering of DNA and RNA contents in Vicia faba root tips. The inhibitory effects of most Bison treatments were found to depend on exposure time dose. Hundred percentage Bison treatment for 24h caused the highest total abnormalities (90.32%) and the lowest content of DNA and RNA (4.63 and 1.98 mgg) respectively compared with respective control 2.12%,9.73 and 3.26 mgg respectively.These findings indicate the mutagenic potentiality of the energy drink Bison.