Author/Authors :
Mart-nez-Carrillo, B. E. Laboratorio de Investigacion en Nutrici ´ on - Facultad de Medicina, Universidad Aut ´ onoma del Estado de M ´ ´exico, Paseo Tollocan - Esquina Jesus Carranza - s/n - Colonia Moderna de la Cruz - C.P. 50180 - Toluca, Mexico , Rosales-Gómez, C. A. Laboratorio de Investigacion en Nutrici ´ on - Facultad de Medicina, Universidad Aut ´ onoma del Estado de M ´ ´exico, Paseo Tollocan - Esquina Jesus Carranza - s/n - Colonia Moderna de la Cruz - C.P. 50180 - Toluca, Mexico , Ram-rez-Durán, N. Laboratorio de Microbiolog´ıa M´edica y Ambiental - Facultad de Medicina - Universidad Autonoma del Estado de M ´ ´exico - Paseo Tollocan - Esquina Jesus Carranza, s/n - Colonia Moderna de la Cruz - C.P. 50180, Toluca, Mexico , Reséndiz-Albor, A. A. Laboratorio de Inmunolog´ıa de Mucosas - Escuela Superior de Medicina - Instituto Polit´ecnico Nacional - Plan de San Luis y D´ıaz Miron - C.P. 11340 - Ciudad de M ´ ´exico, Mexico , Escoto-Herrera, J. A. Laboratorio de Investigacion en Nutrici ´ on - Facultad de Medicina, Universidad Aut ´ onoma del Estado de M ´ ´exico, Paseo Tollocan - Esquina Jesus Carranza - s/n - Colonia Moderna de la Cruz - C.P. 50180 - Toluca, Mexico , Mondragón-Velásquez, T. Laboratorio de Investigacion en Nutrici ´ on - Facultad de Medicina, Universidad Aut ´ onoma del Estado de M ´ ´exico, Paseo Tollocan - Esquina Jesus Carranza - s/n - Colonia Moderna de la Cruz - C.P. 50180 - Toluca, Mexico , Valdés-Ramos, R. Laboratorio de Investigacion en Nutrici ´ on - Facultad de Medicina, Universidad Aut ´ onoma del Estado de M ´ ´exico, Paseo Tollocan - Esquina Jesus Carranza - s/n - Colonia Moderna de la Cruz - C.P. 50180 - Toluca, Mexico , Castillo-Cardiel, A. Departamento de Cirug´ıa Experimental - Universidad Quetzalcoatl de Irapuato - Blvd. Arandas No. 975 Colonia Tabachines - ´ C.P. 36715 - Irapuato - Guanajuato, Mexico
Abstract :
The consumption of sweeteners has increased as a measure to reduce the consumption of calories and thus combat obesity and
diabetes. Sweeteners are found in a large number of products, so chronic consumption has been little explored. The objective of the
study was to evaluate the efect of chronic sweetener consumption on the microbiota and immunity of the small intestine in young
mice. We used 72 CD1 mice of 21 days old, divided into 3 groups: (i) No treatment, (ii) Group A (6 weeks of treatment), and (iii)
Group B (12 weeks of treatment). Groups A and B were divided into 4 subgroups: Control (CL), Sucrose (Suc), Splenda (Spl), and
Svetia (Sv). Te following were determined: anthropometric parameters, percentage of lymphocytes of Peyer’s patches and lamina
propria, IL-6, IL-17, leptin, resistin, C-peptide, and TNF-�. From feces, the microbiota of the small intestine was identifed.Te BMI
was not modifed; the mice preferred the consumption of Splenda and Svetia. Te percentage of CD3+ lymphocytes in Peyer’s
patches was increased. In the lamina propria, Svetia increased the percentage of CD3+ lymphocytes, but Splenda decreases it. Te
Splenda and Svetia subgroups elevate leptin, C-peptide, IL-6, and IL-17, with reduction of resistin. Te predominant genus in all
groups was Bacillus. Te chronic consumption of sweeteners increases the population of lymphocytes in the mucosa of the small
intestine. Maybe, Bacillus have the ability to adapt to sweeteners regardless of the origin or nutritional contribution of the same.
Keywords :
Chronic Consumption , Sweeteners , Microbiota , Immunity , Small Intestine , Young Mice
