Title of article :
Genotyping of Hepatitis C virus isolated from hepatitis patients in Southeast of Iran by Taqman Realtime PCR
Author/Authors :
Naserpour Farivar, Taghi qazvin university of medical sciences - School of Medicine, Cell and Molecular Research Institute - Department of Microbiology, ايران , Johari, Pouran qazvin university of medical sciences - School of Medicine, Cell and Molecular Research Institute - Department of Microbiology, ايران , Nezam, Mohammad Kazem zahedan university of medical sciences, ايران
Abstract :
Objectives: To check TaqMan Realtime PCR in detecting genotypes of hepatitis C virus in Iran.Methods: From July 2007 to April 2009, HCV genotyping was done on 52 patients who were referredto Research Centre for infectious Disease and Tropical Medicine, in Bou-Ali Hospital, Zahedan University of Medical Sciences. All these patients had proven hepatitis C infection.Results: Out of 52 anti HCV positive samples, 28(53.84%) had genotype 1, 2 cases (3.88 %) had genotype 2 , 12 (23.08 %) had genotype 3 and 7 (13.4 %) had genotype 4 . Mixed infection with genotypes 1 and 3 was seen in 3 cases (5.77 %).Conclusion: TaqMan probes for detecting genotyping of HCV were successful in picking genotyping of HCV infection especially those with mixed genotypes.
Keywords :
HCV , Genotyping , Realtime PCR
Journal title :
Journal of the Pakistan Medical Association (Centre) JPMA
Journal title :
Journal of the Pakistan Medical Association (Centre) JPMA
