Author/Authors :
erharuyi, osayemwenre university of benin - faculty of pharmacy - department of pharmaceutical chemistry, Nigeria , imieje, vincent o. university of benin - faculty of pharmacy - department of pharmaceutical chemistry, Nigeria , oseghale, irene o. university of benin - faculty of pharmacy - department of pharmaceutical chemistry, Nigeria , ubhenin, abraham e. federal university lafia - faculty of basic health science - department of biochemistry, Nigeria , falodun, abiodun university of benin - faculty of pharmacy - department of pharmaceutical chemistry, Nigeria , choudhary, m. iqbal university of karachi - hej research institute of chemistry, dr. pajwani centre for molecular medicine and drug research, international centre for chemical and biological sciences, Pakistan
Abstract :
Caesalpinia pulcherrima (Caesalpiniaceae) is an ornamental plant with several ethnomedicinal uses. The present study was designed to investigate the brine shrimp cytotoxicity and insecticidal activity of oil obtained from C. pulcherrima root. The powdered root was extracted with methanol and then defatted with petroleum ether (40-60◦C) to obtain a viscous oil. The oil was investigated for its brine shrimp cytotoxicity and insecticidal activity in vitro. The chemical constituents were identified by Gas chromatography-Mass spectrometry. The oil showed significant lethal effect against Artemia salina (Brine shrimp) with LC50 of 23.85 μg/mL and mild insecticidal activity against Tribolium castaneum and Callosbruchus analis with percentage mortality of 20% and 40% respectively at 1 mg/cm^2. GC-MS analysis identified 37 compounds mainly steroids, terpenoids and fatty acids.
Keywords :
Caesalpinia pulcherrima , chemical constituents , cytotoxicity , insecticidal activity
