Prediction and Correlation of CO2 Solubility in MDEAAqueous Solutions Using the Extended Debye-HiickelModel

Sophisticated analytical methods viz. HPLC and HPTLC which being employed foranalysis, are relatively expensive and hence need for simple analytical methods arises.One such method has been developed and applied for routine determination of acyclovir inpharmaceutical formulations and bulk dosage forms. The method is based on the formationof colored species via reaction of the enzyme horseradish peroxidase with Ferric chlorideand 2, 2’- bipyridine in presence of orthophosphoric acid and finally with the drug tobe estimated namely acyclovir to produce a pink colored chromogen ((lambda)max at 400 nm).Statistical analysis of this method exhibited a molar absorptivity value 105 M^-1 CM^-1, andSandell’s Sensitivity of 0.001912. RSD % of the method was found as 0.0386 indicating that the method is reproducible, based on the principle of absorption visible spectrophotometryfor the determination of acyclovir in pharmaceutical formulations. The method is reliableand can be utilized for the determination of acyclovir in various pharmaceutical formulationsand bulk dosage forms.