Determination of Bicalutamide in Formulation andBiological Fluids Based on Cyclodextrin EnhancedFluorescence

A simple and reliable spectrofluorimetric method has been developed for the determination of a new anticancer agent, bicalutamide in the pharmaceutical preparation and biologicalfluids using fluorescence detection and (beta)-Cyclodextrin as an enhancer. The fluorescence of the product was found to have excitation at(lambda)max 272 nm and emission (lambda) lemi at 328 nm.The method shows high sensitivity with linearity range from 0.1 to 0.35 (mu)g/ml. The lower limit of detection (LOD) was found to be 1.3×10^-5 (mu)g/ml, 4.18 x 10^-3 (mu)g/ml and limit ofquantification (LOQ) was found to be 3.93 ×10^-5 (mu)g/ml, 1.269 x 10^-2 (mu)g/ml in formulationand biological fluids respectively. The different experimental parameters affecting the fluorescence intensity were carefully studied and optimized. The proposed method wasapplied successfully for determination of bicalutamide in the pharmaceutical preparation andbiological fluids. The percentage recovery was found to be 99.5276 ±0.588; 100.367 ± 1.745 for formulation and biological fluids respectively.