Development and Validation of a Reversed Phase HPLCMethod for Simultaneous Determination of Amlodipineand Telmisartan in Pharmaceutical Dosage Form

Abstract:A simple, accurate and precise method for the simultaneous determination of amlodipine and telmisartan in bulk drug and pharmaceutical dosage has been developed by RP-HPLC method. Separation was performed on a 5μm Nucleodur® C18 column (250 х 4.6mm ID) with acetonitrile: phosphate buffer at pH 4.5 (60:40v/v) in isocratic elution in less than 9 min with a flow rate of 1.3 ml min-1. Good sensitivity for all analytes was observed with UV detection at 238 nm. The method allowed quantification over the 1-11 μg ml-1 range for amlodipine and 8-80 μg ml-1 range for telmisartan. The method has been applied, without any interference from excipients or endogenous substances, for the simultaneous estimation of these two compounds in bulk drug and in tablets.