Title of article :
Mediated Electrochemical Study on Microperoxidase-11(MP-11)/ DNA System Film Using a New Mediator
Author/Authors :
Zargazi, M.H islamic azad university - Department of Chemistry, ايران , Baheri, H islamic azad university - Department of Chemistry, ايران , Mohammadi, B islamic azad university - Department of Chemistry, ايران , Letafati, H islamic azad university - Department of Chemistry, ايران
Abstract :
Abstract:Direct redox conversion of microperoxidase-11(MP-11) was studied and demonstrated by cyclic voltammetry on a gold electrode modified by a self- assembled monolayer (SAM) of a new sulfur containing compound (6-Amino-5-Nitroso-4-Hydroxy-1,3-Diaza-5- Cyclohexene-2-Thioone) and DNA film. The results showed a pair of well defined, reversible cyclic voltammetric peaks. It thus obtained at an (MP-11)-S-compound-DNA modified Au electrode. Its formal potential, É0 is 0.224 v for 0.1mol/lit phosphate buffer solution (PBS), pH 7 at a scan rate of 500 mv/s. The formal heterogeneous electron transfer rate constantof MP-11 redox conversion on the S-compound and DNA modified electrode was 2.14 s-1. DNA can be considered as a charge carrier to facilitate electron transfer between the enzyme and electrode.
Keywords :
Microperoxidase , 11(MP , 11) , Deoxy ribonucleic acid (DNA) , Direct electron transfer (DET) , Gold electrode , S , Compound (6 , Amino , 5 , Nitroso , 4 , Hydroxy , 1,3 , Diaza , 5 , Cyclohexene , 2 , Thioone)
Journal title :
Quarterly Journal of Applied Chemical Research (JACR)
Journal title :
Quarterly Journal of Applied Chemical Research (JACR)
