Title of article
Investigation and Determination the Binding Site of Glycyrrhizin of Liquorice to DNA
Author/Authors
Manouchehri, F. islamic azad university - Department of Chemistry, ايران , Nafisi, S. islamic azad university - Department of Chemistry, ايران , Bonsaii, M. islamic azad university - Department of Chemistry, ايران , Abdi, K. tehran university of medical sciences tums - Department of Medicinal Chemistry, تهران, ايران , Varavipour, M. university of tehran - College of Aboreyhan - Department of Irrigation Drainage Engineering, تهران, ايران
From page
45
To page
53
Abstract
Glycyrrhizin(GL), is a triterpenoid saponin found in glychyrrhiza glabra (liquorice). This compound is a frequently used and very effective drug for the treatment of various malignancies. This study was designed to examine the interactions of glycyrrhizin with calf thymus DNA in aqueous solution at physiological conditions. FTIR spectroscopic method was used to determine the ligand binding modes, using constant DNA concentration (6.25mM) and various drug/DNA (phosphate) ratios of 1/240 to 1/1. UV-Vis spectroscopic method was used to determine the binding constants and the stability of glycyrrhizin-DNA and complexes in aqueous solution, using constant DNA concentration of 5.1×10-4 M and various glycyrrhizin concentrations of 5×10-6 - 1×10-4 M. Spectroscopic evidence showed that the interaction of glycyrrhizin with DNA occurred via G, A-T and PO2 group with binding constant of KGL-DNA = 5.7×103 M-1 and DNA remains in B-DNA conformation.
Keywords
DNA , Glycyrrhizin , liquoric , FTIR , UV , Visible spectroscopy.
Journal title
Quarterly Journal of Applied Chemical Research (JACR)
Journal title
Quarterly Journal of Applied Chemical Research (JACR)
Record number
2591790
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