Author/Authors :
Nasir, Azam Ali Veterinary Research Institute - Lahore Cantt, Pakistan , Ashraf, Muhammad Usman Veterinary Research Institute - Lahore Cantt, Pakistan , Kausar, Asma Veterinary Research Institute - Lahore Cantt, Pakistan , Mustafa, Nofil Veterinary Research Institute - Lahore Cantt, Pakistan , Fatima, Zain-ul Veterinary Research Institute - Lahore Cantt, Pakistan , Riaz, Rabia Veterinary Research Institute - Lahore Cantt, Pakistan , Shahzad, Waseem Veterinary Research Institute - Lahore Cantt, Pakistan , Sarwar, Mobeen Provincial Disease Diagnostic Laboratory - Cooper Road - Lahore, Pakistan , Khaliq, Abdul Provincial Disease Diagnostic Laboratory - Cooper Road - Lahore, Pakistan , Hussain, Riaz College of Veterinary and Animal Sciences - The Islamia University of Bahawalpur, Pakistan
Abstract :
Polymerase Chain Reaction (PCR) detected concurrent infection of Clostridium (C.) perfringenstype D and C. chauvoei in samples of three cattle out of five which were submitted to Veterinary Research Institute (VRI) for confirmation of C. chauvoei. The animals had a history of fever, lameness and crepitating sound with death occurring within 48 hours after the onset of clinical signs and seemed to be typical cases of black leg. Furthermore, the traditional methods including clinical examination, necropsy findings, cultural and biological methods are not solely enough for the confirmation of disease and are not sufficient to determine the number of pathogens involved in such cases.
Keywords :
Concurrent infection , PCR , C. chauvoei , Cattle , C. perfringenstype D
