Author/Authors :
Rosyidi, Djalal Laboratory of Animal Product Technology - Faculty of Animal Husbandry - Universitas Brawijaya, Indones , Qosimah, Dahliatul Laboratory of Microbiology Dan Immunology - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Amalia Amri, Indah Laboratory of Microbiology Dan Immunology - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Prasetyo, Dodik Clinical of Animal - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Shodiq Permata, Fajar Laboratory of Pathology Anatomy - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Kaltaria Anisa, Agri Laboratory of Pharmacology - Faculty of Veterinary Medicine - Universitas Brawijaya , Retno Putr ,Lucky Laboratory of Microbiology Dan Immunology - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Ayu Cindyasputri, Nanda Laboratory of Microbiology Dan Immunology - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Wulandari Laboratory of Microbiology Dan Immunology - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Leuricha, Yohana Laboratory of Microbiology Dan Immunology - Faculty of Veterinary Medicine - Universitas Brawijaya, Indonesia , Eka Radiati, Lilik Laboratory of Animal Product Technology - Faculty of Animal Husbandry - Universitas Brawijaya, Indones
Abstract :
Coffee contains high phenols, especially chlorogenic acid which acts as a modulation of the immune
response, and antioxidants. Robusta coffee (Coffea canephora) is a type of coffee with high levels of chlorogenic acid and
high caffeine content which has the potential as an antioxidant so that it can be used as a green coffee extract (GCE).
Green coffee extract is made from roasted coffee beans. This study used 48 layer chickens, consisting of 4 treatments
and 12 replications consisting of negative controls (healthy chickens without coffee supplementation), T1 (coffee
extract dose 500 mg / kg BW), T2 (coffee extract dose 1000 mg / kg BW) and T3 (coffee extract dose 1500 mg / kg
body weight. Data in the form of quantitative duodenum organ histopathology using haematoxylin and eosin staining
and histopathological picture of duodenum were observed using a microscope and analyzed descriptively, test for Nrf2
and HO-1 with flow cytometri the result of robusta coffee (Coffea canephora) extract from lampung had a good effect
at a dose of 500 mg / kg body weight against the relative number of Nrf2, HO-1, and histopathological improvement
in the duodenum. The conclusion of this study shows that robusta coffe e (Coffea canephora) extract has the potential as
an anti-inflammatory and antioxidant in chickens.
Keywords :
Robusta coffee , Intestine , Antioxidant , Green coffee , Inflammation
