Author/Authors :
Yılmaz, Volkan Department of Virology - Faculty of Veterinary Medicine - Kafkas University, Kars, Turkey , Coşkun, Nüvİt Department of Virology - Faculty of Veterinary Medicine - Kafkas University, Kars, Turkey , Kuru, Mushap Department of Obstetrics and Gynecology - Faculty of Veterinary Medicine - Kafkas University, Kars, Turkey , Büyük, Fatİh Department of Microbiyology - Faculty of Veterinary Medicine - Kafkas University, Kars, Turkey , Çelebi, Özgür Department of Microbiyology - Faculty of Veterinary Medicine - Kafkas University, Kars, Turkey
Abstract :
Bovine herpes virus type 1/4 (BoHV-1/4) shows affinity especially to the respiratory and genital system
and can cause economic losses by adversely affecting animal health. The purpose of this study was to identify the
presence of BoHV-1 and BoHV-4 in leukocyte samples using molecular methods. Therefore, leukocyte samples were
collected from 100 cattle with endometritis, not immunized against these infections, in small-scale family farms in the
province of Kars, Turkey. The presence of BoHV-1 and BoHV-4 nucleic acids was investigated with polymerase chain
reaction (PCR) specific for the Glycoprotein C (gC) and Glycoprotein B (gB) gene, respectively. BoHV-1 nucleic acid
could not be detected in any of the leukocyte samples. However, BoHV-4 nucleic acid was detected in 42 cattle with
endometritis. Data obtained from this study and identification of the BoHV-4 specific antigen in leukocyte samples
in the province of Kars proves that the BoHV-4 infection is present in the region and plays an important role in cases
of endometritis observed in cattle.
Keywords :
BoHV-I , BoHV-4 , Endometritis , Kars , PCR
