Author/Authors :
Uddin, Inkeyas Poultry Research and Training Center - Chittagong Veterinary and Animal Sciences University, Bangladesh , Islam, Shafiqul Department of Pathology and Parasitology - Chittagong Veterinary and Animal Sciences University, Bangladesh , Rakib, Tofazzal Department of Pathology and Parasitology - Chittagong Veterinary and Animal Sciences University, Bangladesh , Das, Shubhagata Department of Pathology and Parasitology - Chittagong Veterinary and Animal Sciences University, Bangladesh , Kamaruddin, Kazi Poultry Research and Training Center - Chittagong Veterinary and Animal Sciences University, Bangladesh , Biswas, Paritosh Kumar Poultry Research and Training Center - Chittagong Veterinary and Animal Sciences University, Bangladesh
Abstract :
This study was carried out to isolate the infectious bronchitis virus (IBV) in clinical samples from layer
and broiler breeder farms in Chittagong district of Bangladesh. The samples were collected from 7 broiler and 3 layer
breeder farms experiencing respiratory signs with elevated mortality. The virus was identified from trachea, lung and
kidney tissues in clinically infected cases by embryonated egg inoculation and then RT-PCR. From the dead birds,
a total 200 organ samples were collected and the prepared inoculums (following standard operation procedure) were
injected into 9-11 day old chicken embryo at the rage of 0.2 ml/egg. Total RNA were extracted from collected allantoic fluids that showed characteristic changes in embryo. The results was found that about 34.28% and 28.33% sample
positive embryonic changes in broiler and layer breeder farm, respectively. The suspected allantoic fluid was collected
and performed for RT-PCR and about 62.5% and 52.94 % samples were found positive in broiler and layer breeder
farm, respectively.
Keywords :
IBV , Molecular identification , RT-PCR , Gammacoronavirus , Breeder farm
