Effect of Dilution, Cooling and Freezing on Physical and Biochemical Properties of Semen for Holstein Bull Born in Iraq

Seminal plasma composed of a secretion from the male accessory sex glands and epididymis, which contain many organic and inorganic components that have many effects on semen quality , study the activity of enzymes such as GOT , GPT, ACP and ALP and proteins in the seminal plasma are essential for metabolic process which provide energy for viability, motility and fertility of spermatozoa in relation to the freezability of semen and using this parameter as a valuable tool to evaluate the fertility potential of the male. The objective of this study was to investigate the effect of different steps of processing frozen semen (dilution, cooling and freezing) on certain physical and biochemical properties of semen for Holstein bull born in Iraq. This study was conducted in Artificial insemination center of Abu Ghareeb (Ministry of Agriculture). Semen collected weekly with the help of artificial vagina (AV) and the ejaculates were evaluated physically and a signed poor and good ejaculates for 5 mature bulls. Results showed a significant effect of dilution, cooling and freezing on the physical and biochemical parameters, with gradual significant rise in GOT, GPT, ALP, ACP and total protein concentration during different stages of dilution, cooling, and freezing of semen and the biochemical properties showed higher significant values in poor ejaculate in comparison with good. Physical properties and biochemical properties affected by different steps of freezing for both poor and good ejaculates.