Author/Authors :
Hasan, Kamal Department of Veterinary Microbiology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Rathnamma, Doddamane Department of Veterinary Microbiology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Narayanaswamy, Hogalagere Doddappaiah Department of Pathology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Isloor, Shrikrishna Department of Veterinary Microbiology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Marinaik Chandranaik, Basavegowdanadoddi Institute of Animal Health and Veterinary Biologicals (IAH and VB) - Hebbal, Bengaluru , Kshama, Manayapanda Appaiah Department of TVCC - Veterinary College - Hebbal - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Elattuvalappil, Anuradha Menon Department of Pathology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Yamanappa Mukartal, Shivalingappa Department of Veterinary Microbiology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Singh, Shoorvir Division of Animal Health - Central Institute for Research on Goats (CIRG) - Makhdoom - PO-Farah - Uttar Pradesh, India , Gupta, Saurabh Division of Animal Health - Central Institute for Research on Goats (CIRG) - Makhdoom - PO-Farah - Uttar Pradesh, India , Krishnappa, Sarvesha Department of Pathology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru
Abstract :
Parvoviral gastroenteritis is a highly contagious viral disease which causes severe acute haemorrhagic
enteritis and myocarditis in puppies over the age of 3-4 months. The present study was undertaken to detect the
presence of canine parvovirus (CPV) in fecal samples of diarrhoeic dogs by conventional polymerase chain reaction
(PCR) and Lateral flow assay (LFA) test followed by a comparative evaluation of the diagnostic potential of these tests.
A total of 65 samples from dogs were collected including 48 faecal samples, 13 blood samples, and 4 tissue samples
from different breeds and age group of animals presented to Veterinary College Hospital, Hebbal, Bengaluru. The
animals were showing profuse diarrhoea with foetid odor and blood mixed faeces. Of the sixty five samples subjected
to PCR and LFA, thirty and twenty samples were positive by PCR and LFA respectively. Of the thirty positive
samples, seventeen were positive by both the tests and thirteen samples were positive only by PCR. The sensitivity and
specificity of LFA compared to PCR was found to be 56.6% and 91.18%, respectively. The present study indicates that
LFA is more sensitive than PCR and the assay can be routinely used as a rapid field level test for diagnosing CPV
infections in canines.
