Effect of Bacillus amyloliquefaciens as probiotic on total colonies of bacteria, pH and cellulase activity in the small intestine of domestic chicken

This study aimed to determine the effect of the addition of Bacillus amyloliquefaciensinto drinking water on the total colonies of Bacillus amyloliquefaciens, Lactobacillussp, pH, and cellulase activity in the small intestine of domestic chicken in starter period. This study was used 200 days old of domestic chicken CP 808 kept for 8 weeks. Type of cage used 20 units cage measuring 85 cm x 70 cm x 30 cm which each unit contained 10 chickens. The study used the Completely Randomized Design (CRD) with 4 treatments in 5 replications. Treatment was the addition of B. amyloliquefaciensinto drinking water (without B. amyloliquefaciens, 45 x 109CFU/ml, 65x1010CFU/ml, and 43 x 1012CFU/ml). The variablesobserved were the total colonies of B. amyloliquefaciens, Lactobacillussp, pH, and cellulase activity in the small intestine of domestic chicken. The results showed that addition of B. amyloliquefaciensin drinking water of domestic chicken increased the total colonies of B. amyloliquefaciens, Lactobacillussp, and cellulaseactivity in the small intestine, but did not affect the pH. The highest total colonies (B. amyloliquefaciens and Lactobacillussp) and cellulase activity were found on the addition of B. amyloliquefaciensat a level of 43 x 1012CFU/gram. Meanwhile, the pH intestine added B. amyloliquefacienstended to be lower than without B. amyloliquefaciens.