Title of article :
PEMURNIAN DAN KARAKTERISASI XILANASE Streptomyces sp. SKK1-8
Author/Authors :
Meryandini, Anja Institut Pertanian Bogor - FMIPA Kampus IPB Dramaga - Departemen Biologi, Indonesia , Widhyastuti, Nunuk Institut Pertanian Bogor - FMIPA Kampus IPB Dramaga - Departemen Biologi, Indonesia , Widhyastuti, Nunuk Lembaga Ilmu Pengetahuan Indonesia - Pusat Penelitian Biologi, Indonesia , Lestari, Yulin Institut Pertanian Bogor - FMIPA Kampus IPB Darmaga - Departemen Biologi, Indonesia
Abstract :
Purification and Characterization of Streptomyces sp. SKK1-8 xylanase. Streptomyces sp. SKK1-8 is a xylanase produced bacteria. Purified xylanase has an optimum condition at pH 4.5 and 50°C. The molecular mass of purified xylanase were determined to be 14.4 kDa and 13.4 kDa. The xylanase was capable of hydrolysing p-NP-α-L-arabinofuranoside, p-NP-β-D-xylanopiranoside, p-NP-β-D-glucopiranoside, p-NP-α-D-galactopiranoside. The Km and Vmax values at 50°C measured on Birchwood xylan were 0.101 mg/ml and 0.1796 μmoles/minute/ml.
Keywords :
Xylanase , streptomyces , purified
Journal title :
Makara Journal Of Science
Journal title :
Makara Journal Of Science
