Title of article
Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine
Author/Authors
Wicaksono, Wiyogo P. university of indonesia - Faculty of Mathematics and Science - Department of Chemistry, Indonesia , Ivandini, Tribidasari A. university of indonesia - Faculty of Mathematics and Science - Department of Chemistry, Indonesia , Saepudin, Endang university of indonesia - Faculty of Mathematics and Science - Department of Chemistry, Indonesia , Einaga, Yasuaki Keio University - Faculty of Science and Technology - Department of Chemistry, Japan , Einaga, Yasuaki JST-CREST, Japan
From page
96
To page
100
Abstract
An immunochromatographic strip test based on the complex reaction of antigen-antibody (melamine-antimelamine) was developed for quantitative detection of melamine. Gold nanoparticles (AuNP) were used to form AuNP-labeled antibody, which then acted as a biosensor. Melamine quantification was performed by the determination of AuNP using anodic stripping voltammetry technique with a boron-doped diamond as the working electrode. With sample volume of 100 μL and immunoreaction time of 7 min, the developed immunochromatographic strip test produced a linear calibration curve for melamine concentration range of 0–0.6 mg/L, with detection limit of 0.1 mg/L and RSD of ~5%. Furthermore, negative results were obtained for samples containing cyanuric acid and urea, indicating that the developed immunochromatographic strip test has potential for selective and quantitative detection of melamine.
Keywords
anodic stripping voltammetry , boron , doped diamond , gold nanoparticles , immunochromatographic strip test , melamine
Journal title
Makara Journal Of Science
Journal title
Makara Journal Of Science
Record number
2594703
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