Author/Authors :
Hamidon, Hanisuhana International Islamic University Malaysia - Faculty of Pharmacy - Department of Pharmaceutical Technology, Malaysia , Taher, Muhammad International Islamic University Malaysia - Faculty of Pharmacy - Department of Pharmaceutical Technology, Malaysia , Jaffri, Juliana Md International Islamic University Malaysia - Faculty of Pharmacy - Department of Pharmaceutical Technology, Malaysia , Tg Zakaria, Tg Muhamad Faris Syafiq Faculty of Pharmacy - International Islamic University Malaysia - Department of Pharmaceutical Technology, Malaysia , Sulaiman, Wan MAW International Islamic University Malaysia - Faculty of Pharmacy - Department of Basic Medical Science, Malaysia , Susanti, Deny International Islamic University Malaysia - Faculty of Science - Department of Chemistry, Malaysia , Ichwan, Solachuddin JA International Islamic University Malaysia - Faculty of Dentistry, Malaysia , Zakaria, Zainul A Universiti Putra Malaysia - Faculty of Medicine and Health Science - Department of Biomedical Sciences, Malaysia
Abstract :
Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and antiinflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation
Keywords :
Garcinia xanthochymus , Guttiferae , cytotoxicity , anti , inflammatory , in vitro
