Sumbawa Mare Milk as a Preventive against Inflammation in the Gaster of Inflammatory Bowel Disease (IBD) Animal Model

Inflammatory Bowel Disease (IBD) is one of the chronic inflammatory diseases that occur in the digestive tract and often attacks older animals. Prolonged inflammation may cause digestive organ damage which is characterized by an increase in proinflammatory cytokines. This study was conducted to determine the preventive effect of Sumbawa mare milk on TNF-α level and the number of inflammatory cells in the gastric organs of IBD induced by indomethacin model animal. 20 rats were divided into 5 treatment groups, namely the negative control group (K-), the positive control group (K +) and the preventive group of Sumbawa mare milk with a volume of 0.5 mL / rat (T1), 1 mL / rat (T2) , and 1.5 mL / rat (T3) for 21 days. Groups K +, T1, T2, T3 were induced with indomethacin single dose (dose: 15 mg / kgBW) to make IBD model animal. The parameters observed in this study were TNF-α expression using the Flowcytometry method and the number of inflammatory cells counted in 5 gaster histopathic field of view. Data analysis of the study results was carried out using one way ANOVA (Analysis of Variance) method with a 95% confidence level (p <0.05) and continued with Tukey test. The results showed a decrease in Inflammatory cell numbers in gastric organs in all groups of preventive groups (T1, T2, T3) compared to the positive control group. The T3 group was not significantly different from the K- group. This is in line with the decrease in TNF-α level in all preventive groups (T1, T2, T3) which are significantly different from the K + group but not different from the Kgroup. Conclusions of this study indicate the administration of Sumbawa mare milk in animal models can prevent changes in gastric histopathology, reduce inflammation cells infiltration and reduce TNF-α expression The best volume of administration in this study was 1.5 ml / rat per day.