DeterminationofN-CarbamoylglutamateinRumenFluid

N-carbamoylglutamate (NCG) is a feed additives obtained synthetically from sodium glutamate,whichis a structural analogue of N-acetylglutamate. Current study is aimed at developing ionchromatographic method for the determination of NCG in rumen fluid.Successful determinationwas performed on cation-exchange column and non-suppressed conductivity detection with totalrune time of 11 minutes.Simple one-step cleanup using anionexchange cartridge was used toremove interfering compounds. Method was applied to determine NCG in rumen fluid, which wasobtained from three rumen fistulated dairy cows. Incubated NCGsample inrumenfluidwasassessed at various time intervals (0, 2, 4,6, 12 and 24 h at 39 ºC). Results showed that NCGwasdisappeared at the rate of 7.8, 12.8, 13.3, 14.2, and 17.8 % at 2, 4, 6, 12 and 24 h, respectively. Itis concluded from the results that the procedure is quick and uncomplicated. NCG can be detectedin rumen fluid without lengthy separation using cation-exchange chromatography. This techniquecan be applied for determination of NCG in rumen fluid as well as for practical use in animalsfeed.