The addition of fish Salmon Omega-3 in tris egg yolk diluents on the quality of Simmental bull frozen semen

The sperm plasma membrane is drastically altered by cryopreservation. Therefore, it is necessary to protect the sperm plasma membrane during the cryopreservation process. Omega-3 is a supplement that can protect plasma membrane. This study aimed to compare the quality of Simmental bull frozen semen in Tris Egg Yolk (TEY) diluents with and without omega-3. Semen samples from five Simmental bulls belonged to Lembang Artificial Insemination Center (AIC) werecollected using artificial vaginas.The semen samples were evaluatedimmediatelly(macro and microscopically)after collection. The semen samplesthen divided into two tubes and diluted withTEY or TEYwith Omega-3 (TEYO)diluents respectively. The semen were packed into 0.25 mL straws and equilibratedat 5 °Cfor 4 hours and froze using automatic freezing machine and stored in liquid nitrogen tank (-196 oC) for further evaluation.The quality of frozen semen were evaluatedafter 24 hours of storage. The data wereanalyzedusing independent sample T-test.The resultsshowed that the sperm post-thawing motility in TEY and TEYO diluents were 45.17±1.98%and 47.48±3.55%,respectivelly. No significant differencebetween TEY and TEYO(P>0.05) was found in the sperm motility, individual score, sperm viability as well as membrane integrity. The research concludethat omega-3 supplementation in Tris egg yolk did notimprovethesemen quality of Simmental bull after freezing.