Title of article
Genetic Diversity between Bali Cattle (Bos javanicus) and It’s Hybrids Using Microsatellite Markers
Author/Authors
Jakaria ، J. Department of Animal Production and Technology - Faculty of Animal Science - Institut Pertanian Bogor University , Alwiyah ، A. Goat Research Station , Saputra ، F. Indonesian Research Institute for Animal Production , Baihaqi ، M. Department of Animal Production and Technology - Faculty of Animal Science - Institut Pertanian Bogor University , Noor ، R.R. Department of Animal Production and Technology - Faculty of Animal Science - Institut Pertanian Bogor University
From page
453
To page
460
Abstract
The aim of this study was to evaluate the Bali cattle genetic diversity and hybrid cattle using microsatellite markers. Blood samples (n=192) were collected from various cattle breed, i.e. Bali (n=96), Madura (n=48), and Peranakan Ongole (PO) Kebumen (n=48). The microsatellite locus used for assessing the genetic variation was INRA035, ILSTS006, ETH225, and HEL9, while the genetic profile was described using GenAlEX, Cervus, MEGA6, structure and R programs. As a result, 46 alleles were found at four microsatellite loci studied. The genetic diversity of Bali cattle from Bali island (Ho=0.337) and Nusa Penida island (Ho=0.375) were recorded lower than that of the hybrids, i.e. Madura (Ho=0.747) and PO Kebumen (Ho=0.567) cattle breeds. Specifically, we found 283 bp (locus ILSTS006), 194 bp (locus ETH225), 147 bp and 151 bp (locus HEL9) demonstrating the distinctive alleles for Bali cattle. Also, our experimental data showed that microsatellite markers used allowed us to produce an obvious differentiation of the cattle cluster between Bali cattle and the hybrids, which was meaningful for future cattle breeding program.
Keywords
Bali cattle , genetic diversity , microsatellite marker
Journal title
Iranian Journal of Applied Animal Science
Journal title
Iranian Journal of Applied Animal Science
Record number
2596628
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