Title of article
The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
Author/Authors
Balc-Okcanoğlu, Tuğçe Vocational School of Health Sciences - Near East University, Nicosia, TRNC, Cyprus , Yilma-Susluer, Sunde Faculty of Medicine - Department of Medical Biology - Ege University, Bornova, Izmir, Turkey , Kayabasi, Cagla Faculty of Medicine - Department of Medical Biology - Ege University, Bornova, Izmir, Turkey , Ozme-Yelken, Besra Faculty of Medicine - Department of Medical Biology - Ege University, Bornova, Izmir, Turkey , Gunduz, Gunduz Faculty of Medicine - Department of Medical Biology - Ege University, Bornova, Izmir, Turkey
Pages
5
From page
39
To page
43
Abstract
We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC50 dose and untreated control cells. Expressions of genes related to cell cycle control (CCND2, RB1, ATM, CDC34, CDK5RAP1) were evaluated by qRT-PCR by the LightCycler 480 System (Roche). GAPDH and ACTB housekeeping genes were used for the normalization of gene expressions. IC50 value of CAPE in MCF-7 cells was calculated as 75µM. It was shown that IC50 dose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells.
Keywords
CAPE , MCF-7 , cell line , Cell cycle control genes
Journal title
Molecular Biology Research Communications
Serial Year
2021
Record number
2596673
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