Isolation, identification and characterization of dye degrading bacteria from dyeing industry effluent and degradation process optimization against Novacron Red SB

Water pollution due to the desertion of enormous volume of effluent like synthetic dyes mostly azo dyes from the textile dyeing industries has become an alarming trend in the present world. Biological degradation of synthetic dye is gaining popularity because of its cost effectiveness and ecofriendly procedure. Hence, the present study was aimed to isolate and identify the indigenous azo dye decolorizing bacteria from dyeing industry effluent and to assay their dye decolorization potential in order to use them as an efficient bio-agent for decolorizing and mineralizing toxic azo dyes. Thirty indigenous bacterial colonies were isolated initially from which eight bacterial isolates were selected by further screening method, exhibiting 10% Novacron Red SB decolorization in semi-solidified screening medium. The decolorizer isolates were identified upto species as Bacillus alvei, Bacilluspolymyxa, Corynebacteriumrathayi, Staphylococcus aureus, Zymomonas anaerobia, Bacillusmegaterium,Aerobacteraerogenesand Micrococcus conglomeratuson the basis of their morphological, cultural, physiological and biochemical characteristics. The maximum decolorization by the isolates was achieved with 5% dye concentration at 37°C temperature and pH 9 and peptone as co-substrate. So, this study demonstrates that the selected eight indigenous isolates can be used as efficient biological agent for the removal of toxic industrial novacron dyes by maintaining the above mentioned optimum value of the process parameter.