Author/Authors :
Tugiyono, Department ofBiology - Faculty of Mathematics and Natural Sciences - University of Lampung, Indonesia , Setiawan, Agus Department ofForestry - Faculty of Agriculture - University of Lampung, Indonesia , Rusyani, Emy Center for Marine Aquaculture - Lampung, Indonesia , Gustina, Erlin Department ofBiology - Faculty of Mathematics and Natural Sciences - University of Lampung, Indonesia , Suharso, Department of Chemistry - Faculty of Mathematics and Natural Sciences - University of Lampung, Indonesia
Abstract :
The availability of microalgae(phytoplankton) has an important role in aquaculture.Nannochloropsissp.is amicroalgaethatcanbeusedasthelivefeedfor larvaecultivation ofshrimp, fish and shellfish. In this study,a Nannochloropsissp.isolate obtained fromLampung Mangrove Center waters was cultured by using combination of agriculturalfertilizerasasubstitute forproanalyze fertilizer(Conway). The aimsofthisresearchweretoinvestigatethecelldensity (cell/L),thegrowth rateofcell,and doubling timeofisolatesNannochloropsissp.from thewaters ofLampungMangroveCenter,whichwereculturedintermediately withthe volumeof100L. Thisresearchwasdesigned using completely randomized design (CRD),with 5 treatmentsof agriculturalfertilizer consisted of A:Urea 40,ZA 20,TSP5 ppm,B:Urea40,ZA20,TSP10ppm,C: Urea40,ZA20,TSP15 ppm, D: Urea40,ZA20,TSP20ppm,andE:Conway andvitaminB121ppm (control),with4replications. Theresultsshowedthatt h e treatment of A producedthe highestcell density andthefastestgrowthrate(p<0.050)comparedwiththeothers. Inaddition,thetreatmentofAshowedthefastest doublingtimesignificantly (p<0.050)toB,D,andEtreatments,butitisnot in significantdifferencewiththe treatmentof C(p=0.065).It was concluded that the combinationof the agriculturalfertilizertreatmentAwasthe bestforgrowthrateof Nannochloropsissp.in intermediateculture.
Keywords :
Lampung mangrovecenter , Agricultural fertilizer , Intermediatescaleculture , Nannochloropsissp
