Author/Authors :
Keklik, Muzaffer Kayseri Training and Research Hospital - Clinic of Hematology, Turkey , Köker, M. Yavuz Erciyes University - School of Medicine - Department of Immunology, Turkey , Korkmaz, Serdal Kayseri Training and Research Hospital - Clinic of Hematology, Turkey , Şıvgın, Serdar Erciyes University - School of Medicine - Department of Hematology, Turkey , Kaynar, Leylagül Erciyes University - School of Medicine - Department of Hematology, TURKEY , Çamlıca, Demet Erciyes University - School of Medicine - Flow Cytometry Unit, Turkey , Eser, Bülent Erciyes University - School of Medicine - Department of Hematology, Turkey , Çetin, Mustafa Erciyes University - School of Medicine - Department of Hematology, Turkey , Ünal, Ali Erciyes University - School of Medicine - Department of Hematology, Turkey
Abstract :
Objective: Our aim was to evaluate the contribution of flow cytometry (FC) analysis in patients with a histopathological diagnosis of mantle cell lymphoma (MCL) and to develop a reliable scoring system.Materials and Methods: We assessed the results of FC analysis in peripheral blood or bone marrow samples of 36 patients diagnosed with MCL by histopathological evaluation. Multivariate analysis identified 5 variables associated with MCL: CD23 negativity, CD5/19 common positivity, CD20/22 common bright positivity, FMC7 positivity, and clonal sIg bright positivity.A 5-point scoring system was devised using these parameters.Results: Twenty-five patients (69.4%) scored 5 points, 8 (22.2%) scored 4 points, and 3 (8.4%) scored 3 points. The diagnosis of MCL was confirmed by FC immunophenotyping in 91.6% of the patients.Conclusion: Our results suggest that a quintet diagnostic scoring system, which is considered to be reliable in the diagnosis of MCL, could make a substantial contribution to diagnosis and should be more widely introduced in practice.
