Determination of Median Lethal Dose of Zinc chloride in Wistar Rat

Zinc is one of the most abounded trace elements in the body following iron. Zinc when supplemented in a trace amount participates as a catalytic cofactor of enzymes and regulates various metabolic functions in the body. Excess supplementation of any trace element disturbs the metabolic functions of organs and leads to toxicity. The aim of the work was taken to find out the median lethal dose of zinc chloride (ZnCl2 ) to albino rats via intraperitoneal (IP) route. A single dose of ZnCl2 was dissolved in distilled water (Milli-Q) and administered intraperitoneally at the concentrations of 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 mg/ kg body weight of the experimental animals. Experimental units were observed every 3 hrs before dose administration and later after 6 hrs, 12hrs, 24 hrs and 48hrs for the development of any abnormal behaviors and toxicity symptoms. After 48 hrs, mortalities were estimated in each group in correlation to the total susceptible population. The obtained results were evaluated by the Statistical Probit Analysis Method. The results revealed that the lethal dose 50 (LD50) value within 48 hrs in Albino rats was 57.348 mg/kg b.w. The identification and evaluation of the LD50 against zinc chloride were essential for understanding zinc toxicity because it has been commercially utilized in the form of inorganic zinc salt as a therapeutic agent, dry cell batteries, and for household purpose, thus information about the toxic impacts on ZnCl2 is useful for experimental or toxicological approaches. The in-vitro LD50 evaluations of target chemicals in Wister rats were highly associated with zinc toxicity-related physiological disorders perceptive and therapy.