In Vitro Evaluation of Antibacterial Activity of Antiseptics and Disinfectants Soled in Babil City

In this study (96) swab of clinical pathogenic specimens were collected from Al-Hashimyah General Hospital, by taken from Hospital environment, worker s hands, wards, Hospitalized admitted patients during 2016. Specimens cultured directly on enriched and differential media, colonies purified by streaking, then bacteria diagnosed by culture and cell morphology using gram stained slides, finally confirmed by biochemical tests and Api 20 and Api Staph. So, four bacterial species isolated (Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeroginosa and Escherichia coli). Antibiotics tested using disk-diffusion method, then use two-fold dilutions method to determine MIC. Primary dilutions of chemical agents were prepared using well-diffusion method on Mueller-Hinton agar, also determined MIC. Results of bacterial sensitivity tests to antibiotics shows that both Gram +ve and –ve bacteria resist most antibiotics used against them. On bacterial species they resist most antibiotics used except Strep. pneumoniae and E. coli. Results of determine time needed to kill multi-drug resistant bacteria after exposure to chemical agents shows that most of these agents were active at (20min), then (15min) and not observed at (5min). MIC values of different chemical agents affecting on bacteria shows that these results were higher in Bleach and H2O2 than others, this indicate potency of them in high concentration to inhibit bacterial growth in culture. This study conclude the possible of treating bacteria with these chemical agents used, also the emerge of bacterial resistance to both antibiotics and chemical agents, that face its health and public applications.